Composite

Part:BBa_K3734030:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)


TRE-mCherry-miR21


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 696
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 696
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 696
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because miR21 need to be processed in front of the mature, it need to be insert into a special location.


Source

TRE is from E.coli. The miR21 is from human genomes.

References